Uji Aktivitas Antiinflamasi Ekstrak Etanol Biji Buah Gorek (Caesalpinia bonducella L.) Pada Hewan Coba Tikus Putih (Rattus norvegicus) Jantan

Erniati  Akadir; Sitti Fauziah Noer; Ayu Wandira A.Baso Amri

doi:10.59060/8awjwb12

Authors

Erniati Akadir Universitas Islam Makassar
Sitti Fauziah Noer Universitas Islam Makassar
Ayu Wandira A . Baso Amri Universitas Islam Makassar

DOI:

https://doi.org/10.59060/8awjwb12

Keywords:

Antiinflamasi, Biji gorek, Caesalpinia bonducella L., Jantan, Tikus putih (Rattus norvegicus)

Abstract

Caesalpinia bonducella L. seeds contain bloactive compounds, including flavonoids, terpenoids, phenolics, tannins, and saponins, which have potential anti-inflammatory activity. This study aimed to evaluate the anti-inflammatory activity of the ethanolic extract of Caesalpinia bonducella seeds in carrageenan-induced male white rats (Rattus norvegicus). The extract was prepared by maceration using 96% ethanol. A total of 15 male white rats were randomly divided into five groups: negative control (1% Na-CMC), positive control (diclofenac sodium 0.068 mg/kg BW), and ethanolic extract groups at doses of 200, 400, and 600 mg/kg BW. Paw edema was induced using 1% carrageenan, and edema volume was observed for four hours. Data were analyzed using One-Way Analysis of Variance (ANOVA) followed by Duncan's Multiple Range Test. The ANOVA showed a significant difference among treatment groups (F = 3.798; p = 0.007). Duncan's test indicated that the extract at doses of 400 and 600 mg/kg BW did not differ significantly from the positive control but differed significantly from the negative control. The 600 mg/kg BW dose exhibited the greatest anti-inflammatory activity among the extract-treated groups. In conclusion, the ethanolic extract of Caesalpinia bonducella seeds demonstrated anti-inflammatory activity against carrageenan-induced paw edema, with higher doses showing greater anti-inflammatory effects.

Author Biography

Sitti Fauziah Noer, Universitas Islam Makassar

Peneliti

References

A. Disi, M. Z., Rajih Hi Yusuf, M. F., & Rahman, I. (2024). Aktivitas Antioksidan dan Penentuan Kadar Total Flavonoid Fraksi Daging Buah Pala (Myristica fragrans houtt). Journal Syifa Sciences and Clinical Research, 6(3), 287–300. https://doi.org/10.37311/jsscr.v6i3.28740

Abdulkadir, W., & R.polontalo, F. (2011). Uji efek antiinflamasi ekstrak etanol bunga Rosela(Hibicus Sabdariffa Linn) pada tikus putih jantan(Rattus Norvegicus). Health & Sport, 3(2), 285–362.

Ardiantoi, zahra A., & Rita, E. (2019). Hubungan Pola Makan dan Olahraga Terhadap Kejadian Rheumatoid Arthritis Pada Lansia. Indonesia Journal of Nursng Science and Practice, 2(2), 97–106.

Darmiati, T., Ahmad, K., Feiverin, T., & Viani, A. (2018). Uji Antiinflama Exrak Etanol Kulit Batang Ceremai (phyllanthus acidus L. skell) Tehadap Edema kaki Tikus. Farmakologi Journal Farmasi, 15(1).

Dhyantari, O., Milala, C. T., & Widyaningsih, T. D. (2015). Efek Antiinflamasi dari Eksratk Glukosamin Ceker Ayam. Jurnal Pangan Dan Agroindustri, 3(3), 888–895.

Gadakh, M. j., & Jadhaf, R. S. (2020). Biological potential of caesalpinia bonducella seeds: Review. Journal of Drug DELIVERY and Therapeucs, 10(3), 308–310.

Kumar, A., Nandi, M. K., Kumar, B., Kumar, A., Kumar, R., Kumar, A., & Shing, A. K. (2021). Antiurolithiasis, Antioxidant, Anti-inflammatory, Analgetic, and Diuretic Activity of Ethanolic Extract of Caesalpinia bonducell. Internasional Journal of Pharmaceutical Investigation, 11(3), 306–311.

Lahamendu, B., Bodhi, W., & Siampa, J. P. (2019). Uji efek analgetik ekstrak etanol rimpang jahe putih (Zingiber officinale Rose.var.Amarum) pada tikus putih jantan galur wistar (Rattus norvegicus). PHARMACON-PROGRAM STUDI, FMIPA, UNIVERSITAS SAM RATULANGI, 8(4), 927–935.

Pournaghi, N., Khalighi-Sigaroodi, F., Safari, E., & Hajiaghaee, R. (2021). Bioassay-guided isolation of flavonoids from caesalpinia bonduc (L.) roxb. and evaluation of their cytotoxicity. Iranian Journal of Pharmaceutical Research, 20(1), 274–282. https://doi.org/10.22037/ijpr.2020.112557.13824

Putri, F. S., Arief, M. J., & Rusli, R. (2024). Uji Aktivitas Antiinflamasi Akut Ekstrak Etanol dan Ekstrak Air Daun Telang (Clitoria ternatea L.) dengan Induksi Karagenan. Jurnal Mandala Pharmacon Indonesia, 10(1), 151–156. https://doi.org/10.35311/jmpi.v10i1.467

Ramachandran, A., Muthukrishnan, S., & Maheshwaran, V. (2025). Phytochemical analysis of Caesalpinia bonduc (L.) Roxb. seeds and commercially available seed powder by FTIR and GC-MS techniques. Journal of Drug Research in Ayurvedic Sciences, 10(2), 137–147. https://doi.org/10.4103/jdra_43_24

Sasidharan, S., KP, S. K., S, K. Das, & J, H. N. (2021). caesalpinia bonduc; A Ubiquitous yet Remarkable Tropical lPant Owing Various Promising harmacological and Medicinal Propertes with Special References to the Seed. Medical & Aromatic Plans, 10(7), 394.

Shukla, S., Mehta, A., Mehta, P., Vyas, S. P., Shukla, S., & Bajpai, V. K. (2010). Studies on anti-inflammatory, antipyretic and analgesic properties of Caesalpinia bonducella F. seed oil in experimental animal models. Food and Chemical Toxicology, 48(1), 61–64. https://doi.org/10.1016/j.fct.2009.09.015

Stankov, S. V. (2012). Definition of inflamation, Causes of inflammatio and possible Anti-inflammatory Strategis. The Open Inflammation Journal, 5(3), 1–9.

Suryandari, S. S., Queljoe, E. de, & S.Datu, O. (2021). Anti-Inflammatory Activity test of Ethanol Extract of Sesewanua leaves (Clerodendrum squamatum Valh.) Towards white rats (Rattus norvegicus L.) induced by Carrageenan. PHARMACON-PROGRAM STUDI FARMASI,FMIPA, UNIVERSITAS SAM RATULANGI, 10(3), 1025–1032.