UJI AKTIVITAS EKSTRAK BIJI KOPI ROBUSTA (Coffea canephora) dan daun kersen (Muntingia calabura L.) TERHADAP PERTUMBUHAN BAKTERI Propionibacterium acnes

Mira Bella Apriance; Riza Dwiningrum; Vicko Suswidiantoro; Mida Pratiwi

doi:10.59060/3fe6e905

Authors

Mira Bella Apriance Universitas Aisyah Pringsewu
Riza Dwiningrum Universitas Aisyah Pringsewu
Vicko Suswidiantoro Universitas Aisyah Pringsewu
Mida Pratiwi Universitas Aisyah Pringsewu

DOI:

https://doi.org/10.59060/3fe6e905

Abstract

This study aimed to evaluate the antibacterial activity of robusta coffee bean (Coffea canephora) and cherry leaf (Muntingia calabura L.) extracts against Propionibacterium acnes, as well as to identify their active phytochemical constituents and determine the most effective combination ratio. The research was conducted experimentally in a laboratory setting. Extraction was performed using the maceration technique with 96% ethanol as the solvent, followed by phytochemical screening for alkaloids, flavonoids, tannins, and saponins. The antibacterial activity was assessed using the disk diffusion method with various extract combination ratios of 25%:75%, 50%:50%, and 75%:25%. The inhibition zone diameters were statistically analyzed using a One- Way ANOVA test. The results revealed that both extracts contained secondary metabolites. The combination of robusta coffee bean and cherry leaf extracts at a 25%:75% ratio produced the largest inhibition zone (4.53 ± 1.71 mm), followed by 75%:25% (4.28 ± 2.51 mm) and 50%:50% (3.83 ± 1.24 mm). All were categorized as weak inhibition (<5 mm). Statistical analysis indicated that the data were normally distributed and homogeneous; however, no significant difference was observed among the treatment groups (p > 0.05).

References

Anggreni, N. K. ., & Yowani, S. . (2023). Evaluasi Zona Hambat Berbagai Sediaan Topikal Anti Jerawat Dari Ekstrak Daun Beluntas (Pluchea indica L.). Prosiding Workshop Dan Seminar Nasional Farmasi, 1, 143–157. https://doi.org/10.24843/wsnf.2022.v01.i01.p11

Aryani, D. T., & Riyaningrum, W. (2022). Hubungan Acne Vulgaris (Av) Dengan Kepercayaan Diri Pada Mahasiswa Universitas Muhammadiyah Purwokerto Angkatan 2021. Jurnal Kesehatan Tambusai, 3(3), 434–441. https://doi.org/10.31004/jkt.v3i3.6595

Estikomah, S. A., Amal, A. S. S., & Safaatsih, S. F. (2021). FORMULASI SEDIAAN GEL SEMPROT EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) DAN UJI DAYA HAMBAT TERHADAP BAKTERI Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes. Pharmaceutical Journal of Islamic Pharmacy, 5(1), 36. https://doi.org/10.21111/pharmasipha.v5i1.5705

Fanani Hakim, R., Fakhrurrazi, F., & Masnaini, M. (2020). PERBANDINGAN DAYA HAMBAT EKSTRAK DAUN DAN BUAH JAMBU BIJI (Psidium guajava) TERHADAP AKTIVITAS BAKTERI Enterococcus faecalis. Medika Kartika Jurnal Kedokteran Dan Kesehatan, 3(Volume 3 No 2), 126–138. https://doi.org/10.35990/mk.v3n2.p126-138

Fatimah, S., Prasetyaningsih, Y., & Astuti Carmanyta, S. (2022). UJI EFEKTIFITAS ANTIBAKTERI EKSTRAK DAUN PEGAGAN (Centella asiatica) TERHADAP PERTUMBUHAN BAKTERI Pseudomonas aeruginosa. JFL : Jurnal Farmasi Lampung, 10(2), 92–99. https://doi.org/10.37090/jfl.v10i2.673

Febriani, A., Koriah, S., & Syafriana, V. (2023). Studi Literatur Aktivitas Antibakteri Ekstrak Daun, Kulit Buah, Biji Kopi Arabika (Coffea arabica) dan Robusta (Coffea canephora) Terhadap Berbagai Bakteri Literature Review on Antibacterial Activity of Leaf, Fruit Peel, Seed Extracts of Arabica (Coffea a. Sainstech Farma: Jurnal Ilmu Kefarmasian, 16(2), 94–102.

Isnaini, M., Win Afgani, M., Haqiqi, A., & Azhari, I. (2025). Teknik Analisis Data Uji Normalitas ANOVA. 4(2), 170.

Mauludin, I., Yuwono, H. S., & Dicky Santosa. (2022). Daya Hambat Ekstrak Air Kopi Robusta (Coffea canephora) terhadap Bakteri Propionibacterium Acnes. Bandung Conference …, 2(1), 981–986. https://proceedings.unisba.ac.id/index.php/BCSMS/article/view/1869

Pratomo, G. ., & Dewi, N. . (2018). Tingkat Pengetahuan Masyarakat Desa Anjir Mambulau Tengah terhadap Penggunaan Antibiotik. Jurnal Surya Medika, 4(1), 79–89. https://doi.org/10.33084/jsm.v4i1.354

Rahman, C. A., Santosa, D., & Purwanto, P. (2022). Aktivitas Rimpang Temulawak sebagai Antibakteri Berdasarkan Lokasi Tumbuhnya: Narrative Review. Jurnal Pharmascience, 9(2), 327. https://doi.org/10.20527/jps.v9i2.14007

Ranasatri, A. A., Mahmudah, N., Aisyah, R., & Sintowati, R. (2021). Aktivitas Antibakteri Ekstrak Etanol 70% Biji Kopi Robusta (Coffea canephora) Terhadap Staphylococcus epidermidis dan Salmonella typhi. Biomedika, 13(2), 101–110. https://doi.org/10.23917/biomedika.v13i

Rifda, & Lisdiana, L. (2022). Efektivitas Kombinasi Ekstrak Etanol Daun Kersen dan Kunyit sebagai Antibakteri Propionibacterium acnes. LenteraBio: Berkala Ilmiah Biologi, 11(3), 586–593.

Rishliani, Y. R. (2022). UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN NANAS ( Ananas comosus (L.) Merr.) TERHADAP Propionibacterium acnes SKRIPSI.

Ristanti, A., Suswidiantoro, V., Safutri, W., Daskar, A., & Karim, D. D. . (2024). UJI KUALITATIF EKSTRAK ETANOLIK DAUN KERSEN ( Muntingia Calabura L .) dan BUNGA TELANG ( Clitoria ternatea L .) DARI KECAMATAN PRINGSEWU ,. Journal Pharmacy Aisyah, 136–141.

Sarmira, M., Purwanti, S., & Yuliati, F. N. (2021). Aktivitas Antibakteri Ekstrak Daun Oregano terhadap Bakteri Escherichia coli dan Stapylococcus aureus sebagai Alternatif Feed additive Unggas Staphylococcus Aureus as an Alternative to Poultry feed Additive. 21(1), 40–49. https://doi.org/10.24198/jit.v21i1.33161

Simanjuntak, H. A., Simanjuntak, H., Maimunah, S., Rahmiati, R., & Situmorang, T. S. (2022). Diameter Zona Hambat Antibiotik Amoxicillin dan Tetracycline terhadap Escherichia coli. Herbal Medicine Journal, 5(2), 55–59. https://doi.org/10.58996/hmj.v5i2.52

Tanauma, H. A., Citraningtyas, G., & Lolo, W. A. (2016). Aktivitas Antibakteri Ekstrak Biji Kopi Robusta (Coffea Canephora) Terhadap Bakteri Escherichia Coli. Pharmacon, 5(4), 243–251.